Original article
Endometriosis
Interleukin-17F increases the secretion of interleukin-8 and the expression of cyclooxygenase 2 in endometriosis

https://doi.org/10.1016/j.fertnstert.2011.04.060Get rights and content
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Objective

To examine the effects of interleukin (IL)-17F on the secretion of IL-8 and the gene expression of cyclooxygenase 2 (COX2) in endometriotic stromal cells.

Design

In vitro experimental study using human samples.

Setting

University hospital.

Patient(s)

Endometriotic tissues were obtained from women with ovarian endometriomas undergoing laparoscopic surgery.

Intervention(s)

Endometriotic stromal cells (ESCs) were cultured with IL-17F.

Main Outcome Measure(s)

Concentrations of IL-8 were measured by a specific ELISA, and messenger RNA levels of IL-8 and COX2 were measured by real-time reverse transcription–polymerase chain reaction (PCR).

Result(s)

IL-17F increased the secretion of IL-8 from ESCs, and the effect was inhibited by antibodies for IL-17 receptor A and IL-17 receptor C. Tumor necrosis factor α (TNF-α) synergistically enhanced IL-17F-induced increase in IL-8 secretion from ESCs. The IL-17F increased the gene expression of IL-8 and COX2 in ESCs.

Conclusion(s)

These findings suggest that IL-17F may stimulate the development of endometriosis by up-regulation of IL-8 and COX2.

Key Words

Endometriosis
interleukin-17F
interleukin-8
cyclooxygenase 2

Cited by (0)

T.H. has nothing to disclose. Y.O. has nothing to disclose. M.T. has nothing to disclose. A.S. has nothing to disclose. A.H. has nothing to disclose. K.K. has nothing to disclose. O.Y. has nothing to disclose. Y.H. has nothing to disclose. M.H. has nothing to disclose. Y.T. has nothing to disclose.

Supported in part by Health and Labor Sciences Research Grants from the Ministry of Health, Labor and Welfare of Japan, and Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology.