Experimental animal transplantationCell models: EndotheliumApoptosis in Endothelial Cells by Cyclosporine
Section snippets
Cell Culture
HUVECs were isolated from umbilical cords by collagenase digestion. In brief, after clamping both ends of the umbilical cord, the vein was cannulated with blunt needles, flushed first with air, and then filled with 37°C prewarmed 0.25% trypsin solution for incubation in a humified 37°C incubator for 15 minutes. The collected effluent was centrifuged for 10 minutes and the pellet resuspended in Endothelial Basal Medium-2 Lonza Biologics, Slough, UK) containing 2% fetal bovine serum and
Results
The cell viability in the presence of CsA, was 100.0% ± 0.0% for 0 (controls; 92.7% ± 0.1% for 10 (P < .05 vs control); 84.1% ± 0.1% for 30 (P < .01 vs control); 22.7% ± 0.0% (P < .001 vs control); and 6.5% ± 0.0% for 100 μg (P < .001 vs control).
CsA concentrations of 50 μg/mL showed chromatic condensation of nucleotides and nuclear fragmentation (Fig 1).
Western blot analysis detected p27, p53, procaspase-3, and PARP proteins (Fig 2). CsA unduced p27, reduced procasapase-3, and caused
Discussion
Apoptosis, programmed cell death, is an essential process in development and tissue homeostasis of most multicellular organisms. Deregulation of apoptosis has been implicated in the pathogenesis of many diseases.5, 6, 9, 10 Apoptosis can be induced by stress, radiation, heat, infection, and days nutrition.5, 6, 9, 10 Apoptosis produces characteristic morphological changes including DNA fragmentation, nuclear fragmentation, and chromatin condensation.5, 6, 9, 10 Apoptosis must be controlled,5, 6
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The present research has been conducted by the Research Grant of Dongsan Kidney Institute, Keimyung University in 2009.