Renal transplantationOutcomesThe Impact of Nephrectomy and Renal Transplantation on Serum Levels of Soluble Klotho Protein
Section snippets
Subjects and Methods
Between October 2011 and March 2012, 10 living donors and their concomitant renal transplant recipients were recruited for enrollment in this study. The research protocol was approved by our Medical Ethics Committee; all subjects provided informed consent. Soluble serum Klotho levels were determined 1 day before surgery (baseline), 2 days after surgery, as well as 5 days after surgery using a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) kit (Immuno-Biological Laboratories,
Results
The clinical profiles of the living donors and renal transplant recipients are summarized in Table 1. Eight of 10 recipients had been previously treated with hemodialysis (HD) for a median of 3 years (IR, 2–8.5), and the remaining 2 subjects received transplants prior to commencing HD. Soluble serum Klotho was detectable in both groups. The baseline serum Klotho concentrations among the living donors ranged from 726.4 to 1417.1 pg/mL (median, 909.8 pg/mL; IR, 754.8–1132.4), whereas those of
Discussion
The number of subjects included in the present study was small, implying that this study was underpowered or may have overestimated the evaluation of clinical parameters. Nevertheless, our observations may help to improve understanding regarding the source and kinetics of soluble serum Klotho released by shedding of the enzymatic protein ectodomain.1 The serial data regarding soluble serum Klotho levels obtained from the living donors seemed to support the kidneys as a major source in human
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Serum αKlotho as a Predictor of Graft Dysfunction After Kidney Transplantation
2018, Transplantation ProceedingsCitation Excerpt :In the present study, therefore, serum αKlotho values were measured at a stable state of 1 year after KTx. Similar to a previous report [8,20], serum αKlotho levels were not correlated with eGFR values 1 year after KTx. This association in predialysis patients with CKD is controversial.
Potential application of klotho in human chronic kidney disease
2017, BoneCitation Excerpt :The fact that Klotho-deficient mice and CKD subjects have similar phenotypes also suggests a potential pathogenic role of Klotho deficiency in CKD development and progression [4,24,35–37]. Since the kidney is the main origin for circulating Klotho [6,25] [38,39], it is not surprising that CKD and ESRD patients have low renal Klotho expression and low levels of circulating Klotho. Renal Klotho deficiency in early stages of CKD may be attributed mainly to suppression of Klotho expression rather than loss of viable renal tubules.
αKlotho and Chronic Kidney Disease
2016, Vitamins and HormonesImpact of Renal Transplantation and Nephrectomy on Urinary Soluble Klotho Protein
2015, Transplantation ProceedingsCitation Excerpt :The research protocol was approved by the Medical Ethics Committee of Jichi Medical University, and all subjects provided their informed consent. The amounts of 24-hour urine klotho, as well as the serum sKL levels, were determined 1 or 2 months before surgery (baseline), 2 days after surgery and 5 days after surgery using a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) kit (Immuno-Biological Laboratories, Gunma, Japan) according to the manufacturer's protocol [3,5]. Perioperative renal parameters, including the urine volume and serum creatinine (sCr), also were measured.
Rapid decline of kidney function in diabetic kidney disease is associated with high soluble Klotho levels
2019, NefrologiaCitation Excerpt :Studies performed in patients with CKD stages 1–5 HD, show a decrease of s-Klotho compared to normal subjects, the decrease being more important with the progression of CKD.20,21 Even in the absence of a deterioration of renal function after nephrectomy in living renal donors, a decrease of s-Klotho has been observed.22 Despite the data presented before, in our study patients with eGFR below 60 ml/min, had on average higher s-Klotho levels as compared to those with higher eGFR (without reaching statistical significance).
The Value of Klotho in Kidney Transplantation
2023, Transplantation
Tetsu Akimoto was supported by a grant for pathophysiological research in chronic kidney disease from The Kidney Foundation, Japan (JKFB12-53).